促性腺激素释放激素在不同品种水牛卵巢中的免疫组化定位

Immuno-histochemical localization of gonadotropin-releasing hormone in ovaries of different buffalo varieties

【目的】了解促性腺激释放激素(GnRH)在广西沼泽型水牛与摩拉杂交水牛卵巢中定位表达的异同点,为深入阐明GnRH调节卵巢功能的机制提供理论依据。【方法】采用免疫组化SP染色法对广西沼泽型水牛和摩拉杂交水牛卵巢中GnRH及其受体的分布表达进行对比研究。【结果】两种不同品种水牛卵巢中均存在大量的GnRH阳性细胞,且主要位于颗粒层细胞和黄体粒细胞的胞质内;通过比较显示,两种不同品种水牛卵巢GnRH阳性细胞个数差异极显著(P<0.01),但GnRH阳性细胞大小及其平均光密度的差异均不显著(P>0.05)。【结论】不同品种水牛卵巢的GnRH分布差异可能与繁殖性能有关,但主要分布于巢卵颗粒层细胞和黄体粒细胞的胞质内。

[Objective ]The present experiment was conducted to investigate the similarities and differences in localization and expression of gonadotropin-releasing hormone （GnRH） in ovaries of two different buffalo varieties, viz. Guangxi swamp buffalo and Murrah hybrid buffalo, for studying the mechanism of ovary function regulation by GnRH. [ Methods ] The distribution and expression of GnRH and its receptors in ovaries of buffaloes were compared using immuno-histochemical SP staining. [Results]Results showed the existence of many GnRH positive cells in ovaries of both buffalo varieties, which were mainly located in cytoplasm of stratum granulosum and corpus luteum cells. The quantity of GnRH positive cells in ovaries of different buffalo varieties differed significantly, while the size and average optical density of GnRH positive cells did not show significant differences between two varieties. [Conclusion]Besides stimulating the pituitary secretions of FSH （Follicle stimulation hormome） and LH （Luteinizing hormone） in hypophysis during reproduction, GnRH might play an important role in reproductive organs to regulate the secretion of proteins, production of peptide and steroid hormone by paracrine, self-secretion and neurosecretion, and the regulation of functional activities of these cells and organs. The difference in distribution of GnRH amongst different buffalo varieties might be related with the reproductivity.