摘要
通过金属离子对纤维素酶系中3大组分(β-葡萄糖苷酶、内切葡聚糖酶和外切葡聚糖酶)酶活的影响,探讨金属离子对纤维素酶活影响的作用机理。试验结果表明,金属离子Co2+、Zn2+、Mn2+对内切葡聚糖酶酶活具有促进作用;Co2+和K+对外切葡聚糖酶的酶活具有促进作用;Co2+、Mn2+、Ca2+和Mg2+对β-葡萄糖苷酶具有促进作用,来源不同的纤维素酶,因其酶系组分不一样,酶促离子对纤维素酶的促进作用有所差异。
The study of the three major components （ β- glucosidase, endo - 1,4 - [3 - D - glucanase and exo - 1,4 - β-D -glucanase） enzyme activity was influenced by the metal ions. The results showed that Co2＋ , Zn2 ＋ , Mn2＋ were promoted to internal endoglucanase activity, Co2 ＋ and K ＋ were promoted to External endoglucanase activity, Co2 ＋ , Mn2 ＋ , Ca2 ＋ and Mg2 ＋ were enhanced the β- glucosidase activity. Because of its different component, the activity of enzymes cellulose from different sources was found to be dissimilar under the same metal ions concentration.
出处
《安徽农学通报》
2011年第5期27-28,30,共3页
Anhui Agricultural Science Bulletin
基金
芜湖职业技术学院自然科学研究项目(WZ[2010]ky04)
