高粱DNA导入小麦“矮早8”引起后代变异的研究及SSR分析

Study on Character Variation Induced by Broomcorn DNA Intrducing into the Wheat " Aizao8" and Assessment of Genetic Diversity of the Descendant by SSR Markers

将高粱"抗5"DNA通过花粉管通道导入小麦品种"矮早8",其后代在株高、蜡质层、芒型、穗型、旗叶、成熟期等几个方面发生大量变异。通过对变异后代系统选择得到多个稳定的变异种质系;通过对"矮早8"、D8-1、D8-4、高粱"抗5"基因组DNA的SSR分子标记检测,引物xgwm6、xgwm148、xgwm295、xgwm328、xgwm257检测出了较丰富的多态性DNA片段,其中xgwm6、xgwm257检出了高粱"抗5"DNA特有而"矮早8"没有的特异带型,进一步说明了D8-1、D8-4是由高粱"抗5"DNA片段嵌入"矮早8"基因组DNA变异而来。

There are many descendants had been mutated in plant height.waxiness .awn .Ear type.Flag leave .maturation by importing broomcorn DNA of ＂kang5＂ into the wheat ＂Aizao8＂ through the Pollen- tube Pathway~ We have own many unaltered germplasm line by Subsequent continuous selection in the variational descendants ; Detect the DNA of＂ aizao8＂ D8 - 1 .D8 -4.broomcorn＂ kang5＂ Using SSR markers, There are many differential fragments have been found by the Prime xgwm6 ,xgwm148 、 xgwm295, xgwm328, xgwm257, Especially xgwm6, xxgwm257, they have been found the differential fragments in the DNA of D8 - 1 and D8 - 4 that belong to the hroomcorn DNA of ＂kangS＂ only, It further attest that D8 - 1 and D8 -4 was come from ＂AizaoS＇＂ that was inlaid the fragments of broomcorn DNA of ＂kang5 ＂.