摘要
建立高效液相色谱法测定辣椒样品中Nonivamide(合成辣椒碱)的方法。样品用甲醇-四氢呋喃(1∶1)混合溶液超声提取,以甲醇-水(60∶40,其中每升流动相中含25mmol硝酸银和2.5mL冰醋酸)为流动相,在280nm处测定。线性方程为Y=7180.5C+215.6(r=0.9995),Nonivamide在0.28μg/mL～27.68μg/mL范围内呈良好的线性关系,回收率为96.72%。在天然生长的辣椒中含有Nonivamide,但其含量未超过辣椒素类物质总量的2.0%。
A method was developed for determining nonivamide in Capsicum annuum L. Samples were extracted with methanol/tetrahydrofuran （ 1：1 ） by ultrasonic extraction, eluted with methanol/water （60：40） which 1 L contained 25 mmol AgN03 and 2.5 mL acetic acid, and measured at 280 nm. The linear equation of nonivamide was Y=7 180.5C＋215.6 （r=0.999 5 ） and has good linear relationship range from 0.28 μg/mL to 27.68 μg/mL, the recovery was 96.72 %. Nonivamide occurs naturally in Capsicum annuum U, but its content is less 2.0 % than capsaicinoids.
出处
《食品研究与开发》
CAS
北大核心
2011年第2期115-117,共3页
Food Research and Development
基金
广西教育厅项目(200911MS135)
