咬合创伤诱导海马区星形胶质细胞的上调

Up-regulation of astrocytes to occlusal trauma in rat hippocampus

目的:探讨咬合创伤后海马区星形胶质细胞的变化及意义。方法:通过将大鼠左侧上颌第一磨牙咬合抬高0.5mm造成咬合创伤动物模型,应用免疫荧光染色观察咬合创伤后1、3、7d、2w、4w,5w时星形胶质细胞在海马区的分布和表达变化。结果:正常大鼠海马区仅见少量星形胶质细胞,7d组星形胶质细胞数开始增加,4W时达到高峰,5W时阳性细胞数开始下降。结论:咬合创伤引起了海马区星形胶质细胞的数量增加,海马结构参与了咬合创伤反应,其CA1、CA3区与咬合创伤所致口颌面痛及痛情绪调控关系密切。

Objective： To investigate the expression and the role of astrocytes in hippocampus during occlusal trauma. Methods： The occlusal surface of the left first maxillary molars were raised 0.5mm with the square steel. Immunofluoresence histochemical was used to examine the distribution of astrocytes in rats＇ hippocampus on 1, 3, 7d, 2w, 4w, 5w aider experimental occlusal trauma. Results： Astrocytes in the hippocampus of control group occasionally had GFAP-IR. Comparing to the control group, the number of GFAP-positive astrocytes were increased during the occlusal trauma to a different extent and in a time-dependent manner. The increase of GFAP immnnoreactivity was detected at 7d after occlusal trauma, reaching the maximum at 4 week. Conclusion： The astrocytes in rats hippocampus were highly active during occlusal trauma. The astrocytes in hippocamal formation, especially CA1 and CA3 were involved in orofacial pain and pain relating emotional activities induced by occlusal trauma.