实时荧光定量PCR微卫星分析技术检测少突胶质细胞肿瘤染色体1p、19q和10q杂合性缺失

Loss of Heterozygosity in Oligodendroglioma by Real-time Quantitative Polymerase Chain Reaction-based Microsatellite Analysis

目的建立检测脑胶质瘤染色体1p、19q和10q杂合性缺失(LOH)的实验室新方法。方法采用Taqman探针法对38例少突胶质细胞肿瘤标本进行染色体1p、19q和10q LOH检测。结果 38例少突胶质细胞肿瘤染色体中有25例(65.7%)发生1p LOH,26例(68.4%)发生19q LOH,5例(13.2%)发生10q LOH。结论实时荧光定量PCR微卫星分析技术快速、准确性高,可以用于脑胶质瘤标本染色体LOH的检测。

Objective To set up the new lab examination method for 1p,19q and 10q loss of heterozygosity（LOH） in glioma.Methods Thirty-eight cases of oligodendroglioma were enrolled into the study.Real-time quantitative polymerase chain reaction-based microsatellite analysis was performed on tumor tissues in order to study the status of chromosomes 1p,19q and 10q.Results Among the 38 cases of oligodendroglioma,25 cases（65.7%） showed 1p LOH,26 cases（68.4%） showed 19q LOH,while 5 cases（13.2%） showed 10q LOH.Conclusion Real-time quantitative polymerase chain reaction-based microsatellite analysis is a rapid and specific for detecting LOH in glioma tissues.