献血者初筛和复检ALT测量值的相关性分析

Correlation Analysis of the ALT Measurements between Donors Screening and Rechecking

目的探讨献血者初筛和复检ALT测量值的相关性，为献血者初筛ALT上限值界定提供一个可参考的理论依据。方法初筛采用半自动生化分析仪使用一种试剂进行测定，复检采用全自动生化分析仪，用两种试剂进行测定，均为速率法。对4404份标本的测定结果经对数转换后使用SPSS13．0软件SH，GIP=0．935进行统计学分析。结果3次ALT测定结果相比，P〈0．05。而2次复检结果差异无统计学意义（P〉0．05）。初筛ALT测量值x和2次复检测量值y1和Y2的线性回归方程及相关系数分别为：lgYl=0．124＋0．868lgX，r=0．901；lgY2=0．116＋0．8781gX，r=0．893；依据回归方程推导出对于复栓试剂1和2，初筛ALT水平分别控制在31U／L和30U／L以下，有95％的可能使2种试剂的复检ALT水平不超过40U／L。结论通过统计控制建立了该地无偿献血者初筛上限参考值，最大限度地避免血液浪费，同时也要采取适当的措施保证充足的血源。

Objective To discuss the correlation of the ALT measurements between donors screening and rechecking and provide a reference for the theory basis of ALT upper limit cutoff. Methods A reagent was used to determine ALT in a initial screening by a semi-automatic biochemical analyser. The ALT re-inspection was determined with two reagents by a automatic biochemistry analyser. The rate method was used. The measured results of 4 404 specimens were analysed after conversion by logarithmic using the statistical analysis software SPSS 13.0. Results 3 times ALT determination result were compared （P^0. 05）. 2 times re-assessment results were compared （P^0. 05）. The liner regression equation and cor- relation coefficient between the ALT screening measured values X and 2 times rechecking（Y1 ,Y2） respcetively were ：lgY1 =0. 124＋0. 8681gX,r=0. 901 ;lgY2=0. 116＋0. 8781gX,r=0. 893. According the regression equation,it was deduced theearly screeing ALT should be respectively controlled below 31 U/L and 30 U/L. 95% of ALT reinspction does not exceed 40 U/L. Conclusion ALT early screeing reference measurement in local blood donors was established through the statictical controlling.