EDTA-K2对溶血样本胰岛素测定的作用研究

Effect of EDTA-K2 Investigate on the Detection of Insulin in Hemolytic Sample

目的探讨EDTA—K2是否减弱溶血对胰岛素测定的影响。方法制备10例溶血样本（溶血程度2g／L），每例分为3份（500μl／份），分别加入0，3．8，7．6mg的EDTA—K2，测定在室温放置0，1，2h的胰岛素浓度，每例以其相应未溶血样本为对照。结果溶血与未溶血样本比较，各时间点所测胰岛素浓度均显著降低（P〈0．01），而溶血样本加入7．6mgEDTA—K2则未发生以上改变，加入3．8mgEDTA-K2，只有放置2h的胰岛素浓度仍然显著低于对NN（P〈0．05）。结论EDTA—K2可有效防止溶血对胰岛素测定的影响，其效果与样本中EDTA—K2含量有关。

Objective To investigate the influence of EDTA-K2 on the detection of insulin in hemolytic sample. Methods Ten free-hemolytic and corresponding hemolytic （2 g/L） sample post-treated were collected in this study. Every hemolytic sample was divided into three tubes （500 t^l per tube）. In the three tubes,add 0,3.8 and 7.6 mg EDTA-K2 respectively. The insulin levels of all samples were measured at 0,1 and 2 hour at room temperature. Results The level of insulin in the hemolytic sample were significantly lower than that of free-hemolytic sample at every time assay （P〈0.05）. After treated by 7.6mg EDTA-K2,no significant difference was found between the hemolytic and free-hemolytic sample in the level of insulin at every time assay. In the hemolytic sample contained 3. 8 mg EDTA-K2 ,only the insulin level at 2 hour was signif- icantly lower that of control. Conclusion The impact of hemolysis on detection of insulin may be prevented by the right amount of EDTA-K2.