正交试验优选重连口服液的提取工艺

Optimizing extraction process of Chonglian oral solution by orthogonal test

目的优选重连口服液最佳提取工艺。方法水提取工艺以浸膏得率、重楼皂苷、Ⅰ和连翘苷的含量为考察指标，醇沉工艺以重楼皂苷Ⅰ和连翘苷的含量为考察指标，分别采用正交试验法对其提取工艺条件进行优选。结果重连口服液水提最佳工艺条件为加水煎煮3次，每次10倍量水，煎煮1h，醇沉最佳工艺条件为浓缩至相对密度1．10（80℃热测），加乙醇使药液乙醇浓度达到80％，静置24h。结论优选的提取工艺合理，稳定性好，可为工业生产提供理论依据。

Objective To optimize Chonglian oral solution extracting craft. Methods With the obtaining rate of extract and the total content of the Pariphyllin Ⅰand Phillyrin presented in the extract as the indexes for the water extraction process, and with the total content of the Pariphyllin Ⅰ and PhiUyrin presented in the extract as the indexes for the alcohol deposition process, orthogonal design was used to optimize the conditions for the extraction process respectively. Results The optimal conditions for the water extraction of Chonglian oral solution was as following： to add water 10 times, decocting 3 times, 1 hour each time. The optimal conditions for the alcohol deposition of Chonglian oral solution was as following： concentrated for the relative density to 1. 10 （ 80℃ hot test）, cold, add ethanol to the solution for the ethanol content of the solution reached 80%, static settlement for 24 hours. Conclusion The extracting method is reasonable, stable, and suitable to industrialized producting.