硫堇-牛血清白蛋白氧化还原复合膜修饰葡萄糖生物传感器的研究

A bienzymic biosensor based on thionine-the bovine serum albumin redox composite membrane for the detection of glucose

通过交联法和自组装法制备了一种双酶型葡萄糖生物传感器。首先以牛血清白蛋白-戊二醛为交联剂以实现对辣根过氧化物酶(HRP)的固载,再利用凝集素-糖蛋白的识别作用将葡萄糖氧化酶(GOD)分子组装到电极表面,制得双酶型的葡萄糖生物传感器。采用原子力显微镜(AFM)考察了复合膜的性质,同时采用循环伏安法和计时电流法考察了该传感器的电化学行为,研究了该修饰电极对葡萄糖的催化还原作用。在优化的实验条件下,该传感器在3.0×10-6～2.4×10-3mol/L内呈现良好的线性,检测下限为8.2×10-7mol/L。

A bienzymic biosensor was prepared by means of cross-linking and self-assembly.Firstly,a solution of bovine serum albumin（BSA） containing horseradish peroxidase（HRP） is coated on the gold electrode in such a way that the first enzyme membrane is achieved.Then,by the use of sugar-lectin biospecific interactions,the glucose oxidase（GOD） was attached on the electrode surface between concanavalin A（Con A） and sugar chains intrinsically.The morphologies of composite films were characterized by atomic force microscopy（AFM）.At the same time,the electrocatalytic behavior of the electrode was investigated by cyclic voltammetry and chronoamperometry.The biosensor displayed an excellent electrocatalytical response for detection glucose and the linear range of 3.0×10-6～2.4×10-3 mol/L,with a detection limit of 8.2×10-7 mol/L.In addition,the modified electrode presented good sensitivity,selectivity and stability.