氯化胆碱浸种对菠菜种子萌发·幼苗某些生理特性的影响

Effects of Seed Soaking in Choline Chloride Solution on Seeds Germination and Some Physiological Characteristics of Spinach Seedlings

[目的]用不同浓度的氯化胆碱(CC)溶液浸种后,研究CC对菠菜种子萌发及幼苗某些生理特性的影响。[方法]测定了种子萌发率、发芽指数、蛋白质、叶绿素和可溶性糖含量、抗氧化酶活性、丙二醛含量和超氧阴离子的产生速率。[结果]CC溶液浸种后能提高种子萌发率和发芽指数,提高幼苗叶片中可溶性糖、可溶性蛋白质和叶绿素含量,超氧化物歧化酶、过氧化氢酶和过氧化物酶的活性均有所提高,而超氧阴离子的产生速率和丙二醛含量明显低于未经CC处理的。[结论]各浓度的CC溶液浸种以25 mg/L浸种效果最好。

[Objective] The spinach seeds were soaked with choline chloride（CC） solution of different concentration,and the effects of CC on seeds germination and some physiological characteristics of spinach seedlings were studied.[Method] The germination rate and germination index,soluble protein,solube sugar,chlorophyll content,antioxidant enzyme activity,production rate of O2· and MDA content were measured.[Result] CC could increase germination rate and germination index of spinach seeds.It also could increase soluble sugar,soluble protein and chlorophyll content of spinach seedlings.The superoxide dismutase,the calatase and the peroxidase activities also was increased after soaking with CC solution.Moreover,the production rate of O2· and MDA content decreased markedly contrasting with spinach seedlings without CC treatment.[Conclusion] The effects on seeds germination and some physiological characteristics of spinach seedlings were best after treatment by CC with the concentration of 25 mg/L.