喉癌组织转化生长因子-α及其受体mRNA定量分析的意义

Quantitative analysis of TGF-α and EGFR mRNA in laryngeal carcinomas

为了探讨生长因子自分泌刺激环路在喉癌发生发展中的作用。本研究采用逆转录聚合酶链式反应技术（RT－PCR），以β-actin为内参照，测定喉癌、癌旁正常喉粘膜及声带息肉组织转化生长因子-α（TGF-α）和表皮生长因子受体（EGFR）mRNA水平。结果显示，喉癌和癌旁组织TGF-α和EGFRmR－NA水平明显高于声带息肉组织（P＜0．05），而喉癌组织TGF－αmRNA水平又高于癌旁组织（P＜0．05）。表明TGF－α及其受体在喉癌的极早期，甚至尚未出现组织表型改变时，就可构成自分泌刺激环路，而且TGF－αmRNA表达水平的进一步上调在触发癌变中有重要作用。

To elucidate the stimulating role of the growth factor autocrine loop inthe carcinogenesis and development of laryngeal carcinomas,the Reverse Transcription Polymerase Chain Reaction technique (RT-PCR) was applied with β-actin as internalstandard,TGF-α and EGFR mRNA in laryngeal carcinoma,microscopically normal laryngeal mucosa adjacent to the tumor and vocal cord polyps were quantitatively examined. The mRNA indexes of both TGF-α and EGFR in laryngeal carcinomas and adjacentmucosas were significantly higher than those in vocal cord polyps (P<0.05),and theindex of TGF-α mRNA in laryngeal carcinomas was then significantly higher than inmucosas adjacent to the tumor (P<0. 05). Result showed that the autocrine systemconsisted of TGF-α and EGFR might play an important role in the very early stage of laryngeal carcinoma,or even when the tissue phenotypic alteration does not occur. The elevated TGF-α mRNA level might trigger a switch from genotypic alterations to phenotypic alterations. The results were expected to be a laboratory basis for the preventivechemotherapy of laryngeal carcinomas.