视网膜下液维A酸含量测定及临床意义

Analysis of retinoic acid in the subretinal fluid from the rhegmagogenous retinal detachment

目的探讨视网膜下液中维Ａ酸含量与孔源性视网膜脱离眼部病变程度的关系。方法高效液相色谱法测量视网膜下液维Ａ酸的含量。结果（１）孔源性视网膜脱离患者视网膜下液中维Ａ酸的含量随增殖性视网膜玻璃体病变（ＰＶＲ）程度的加重而增加（Ｐ＜０．０５），而血清中维Ａ酸的含量与ＰＶＲ程度无明显相关（Ｐ＞０．０５）。（２）口服维生素Ａ５万ｕ／次，３次／天，４天以上视网膜下液中维Ａ酸的含量显著高于服药天数在２～３天的患者（Ｐ＜０．０５），而血清中维Ａ酸的含量口服维生素Ａ组与未服维生素Ａ组之间有显著性差异（Ｐ＜０．０５）。结论（１）视网膜脱离患者视网膜下液中含有维Ａ酸，其含量随ＰＶＲ程度的加重而增加。（２）服用维生素Ａ可提高ＳＲＦ中维Ａ酸的含量，增加视网膜色素上皮细胞对其的利用度，可望成为一种防治ＰＶＲ的有效药物。

Objective To investigate rhe relationship between the rentinoic acid(RA)in the subretinal fluid(SRF)and serum from the rhegomatogenous retinal detachment and the extent of the rhegomatogenous retinal detachment.Methods High performance liquid chromatography(HPLC). Results 1.The contents of the retinoic acid in the SRF increased with the grade of rhe proliferative vitreoretinopathy(PVR).( P <0.05),but those in rhe serum were not related to the grade of PVR.2.The contents of the retinoic acid in the SRF from the patients treated with 50000U oral vitamin A for less than 4 days were higher than in 2～3days( P <0.05),but there was significant difference between those in the serum from the patients who received oral vitamin A and received no vitatim A.Conclusion 1.The SRF from the rhegmatogenous retinal detachment contained RA,whose contents increased with the grade of PVR.2.Oral vitamin A could increase the contents of RA in the SRF,and RA might be useful as an effective in eyes with PVR.