结核病五项实验检测方法的对比分析

The evaluation of 5 laboratory detection methods on the diagnosis of tuberculosis

目的 对实验检测方法进行对比分析，探讨对结核病诊断的价值。方法 选健康人５３ 名、肺结核７０ 例及非结核肺部疾患３０ 例，采用痰涂片、培养、结明试验、酶联免疫法及聚合酶链反应检测进行对比分析。结果 涂片、培养、酶联免疫法特异性高，但敏感性低于５０ ％ ，其次为聚合酶链反应法，结明试验特异性与敏感性分别为９８１ ％ 及６４２ ％ 。结论 结明试验具有特异性高、简便、快速等特点，是结核病诊断的一种有力辅助手段。

Objective To analyse and co mpare 5 laboratory dectection methods and try evaluate the effect ondiagnosis of tuberculosis . Methods 53 health persous .70 pulm .tuberculosis cases and 30 cases with non tuberculo sis disease were studies and using sputu m sm ear ,culture , Myco Dot test , P C R, E L I S A assay ,all results were co m pared . Results The studies sho wed that smear ,culture , E L I S A had high sensitivity but the specificity are belo w50 % ,and then the P C R assay . Conclusion Myco Dot have high specificity ,simple and convenient ,rapid superiori ty ,this assay may be the m ore valuable auxiliary test on diagnosis of tuberculosis .