系统性硬化病血管内皮活性因子测定的临床意义

THE CLINICAL SIGNIFICANCE OF DETERMINATION OF PLASMA ENDOTHELIAL ACTIVE FACTORS IN PATIENTS WITH SYSTEMIC SCLEROSIS

目的:从蛋白分子水平探讨血管内皮活性因子同系统性硬化病(SSc)的关系。方法:选择42例SSc病人,采用放射免疫测定法检测血浆内皮素(ET)、血栓素B_2(TXB_2)、6-酮-前列腺素F_(1α)(6-K-PGF_(1α)),采用Griss方法检测血浆一氧化氮(NO)水平,采用酶联免疫法检测细胞间粘附分子-1(ICAM-1)、p选择素(P-S)。结果:所有SSc患者均存在高ET、TXB_2、ICAM-l、P-S血症,存在低NO、6-K-PGF_(1α)血症。结论:ET、NO等血管内皮细胞活性因子同SSc的发生发展密切相关,血浆内皮细胞活性因子的检测有助于SSc发病机制的探讨,有助于SSc的疗效观察。

investigate the relationship between systemic sclerosis and endothelins (ET), nitric oxide (NO) as well as other endothelial active factors. Methods: The plasma ET, NO, thromboxane B2 (TXB2), 6 - keto - Prostaglandin F1α(6 - K - PGF1α), Intercellular Adhesion Molecule - 1(ICAM - 1) were respectively determined by density gradient method, radioimmunoassay and enzyme immunoassay method in 42 patients with SSc. Results: All SSc patients had high levels of ET, TXB2, ICAM - 1 and had low levels of NO, 6 - K - PGF1α. Conclusion: The SSc patients have endothelial injuries. It is useful to investigate the pathogenesis of SSc and to judge the therapeutic effects through observations on vascular endothelical active factors.