内皮素、一氧化氮在烧伤后肾脏血流调节中的作用

The significance of endothelins, nitric oxide on the regulation of renal blood flow in burnt rats

目的:研究内皮素(ET)、一氧化氮(NO)在烧伤后肾脏血流调节中的作用。方法:动态观察大鼠30%度烧伤后血浆及肾脏ET、NO含量及肾脏血流量变化,应用ET受体拮抗剂和外源性NO载体观察其对烧伤大鼠肾脏血流量的影响。结果:大鼠烧伤后肾脏血流量明显下降,伤后血浆及肾组织ET、NO含量明显升高,由于ET升高幅度相对较大,ET/NO比值也显著增加,肾组织ET、ET/NO改变与肾脏血流量的降低呈显著负相关;伤后在一定的液体复苏下应用两种ET受体拮抗剂、NO供体在一定程度上明显使肾血流量增加,其中同时拮抗ETA、ETB较单独拮抗ETA效果好,ET受体拮抗剂和NO供体配合使用效果更佳。结论:烧伤后由于肾脏ET的增加和内源性NO相对不足,导致肾脏血管收缩造成肾脏血流量下降,拮抗ET的作用和(或)

Objective:To elucidate the significance of endothelins, nitric oxide on the regulation of renal blood flow in burnt rats. Methods:By use of a 30% TBSA (total body surface araa) Ⅲ degree burnt rat model, we detected the dynamic changes of the contents of endothelins and nitric oxide in postburn plasma and kidney. Meanwhile, the effects of endothelin receptor antagonists and NO donor on renal blood flow in burnt rats were observed. Results:The renal blood flow decreased sharply in burnt rats, accompanied by the increases of endothelins and nitric oxide in plasma and kidney. The ratio of ET/NO in kidney elevated significantly. The changes of ET and the ratio of ET/NO were significantly correlated with renal blood flow. Administration of endothelin receptor antagonists (PD 147953 , PD 145065 ) and NO donor (SIN 1) was demonstrated to be able to reverse the decrease of blood flow in kidney postburn. Furthermore, better outcome could be achieved by blocking both endothelin receptors than by interfering with only ETA, and even much better result could be anticipated if NO donor was given together. Conclusion:The pathological alterations of endothelins, nitric oxide contributed to the regulation of renal blood flow postburn.