运用变性PAGE和荧光检测技术对广州汉族人群六个STR位点的研究

PCR-based DNA analysis of vWA31A, TH01, F13A01, FESFPS, TPOX and CSF1PO

运用STR-PCR、变性聚丙烯酰胺凝胶电泳结合荧光DNA自动检测技术对广州汉族人群6个STR位点(vWA31A、TH01、F13A01、FES、TPOX和CSF1PO)等位基因频率和基因型频率进行了调查,并与其它人群的等位基因频率进行了对比。所有位点的结果均符合Hardy-Weinberg平衡,且各位点等位基因间无相关性。本方法可靠、灵敏,所得的等位基因频率等数据可为汉族人群法医个人识别、亲子鉴定及遗传学研究提供基础数据。

The allele and genotype frequencies of 6 tetranucleotide STR loci were investigated in a sample of 132 unrelated individuals from Chinese Han population. The PCR products were analyzed on 6% denaturing PAGE and detected using fluoreseently labeled primers in an automated 377 sequencer(PE). All loci meet Hardy- Weinberg equilibrium. There was no random association of alleles among the 6 loci. The allele frequencies were compared with other population databases. Except locus vWA31A, the observed heterozygosity at other 5 loci was significantly lower than that reported in Caucasian and Black population studies. The calculated DP=0.99999, PE=0.9708, pM=1.059×10^(-5). The allelic frequency data can be used in forensic identification and paternity testing.