拟南芥春化相关基因VRN2的克隆及其植物表达载体的构建

Cloning Vernalization-Related Gene VRN2 and Construction of its Plant Expression Vector in Arabidopsis thaliana

本文利用特异性引物,从拟南芥RNA中提取春化相关基因VRN2 cDNA序列,GenBank登录号AY063047。该基因序列大小为1 354 bp,编码区为1 323 bp,编码氨基酸441个。将克隆片段插入中间载体pBPF?7,经PstI酶切回收带有P35S启动子和nos终止子片段,连接pBI121载体,构建VRN2基因的植物表达载体。

In this paper,the vernalization-related Gene VRN2 cDNA from RNA of Arabidopsis thaliana L.,GeneBank accession number AY063047 was successfully cloned.The cDNA sequence of VRN2 was 1 354 bp and its region in encoding was 1323 bp.The protein deduced from VRN2 contained 441 amino acids.The cloned cDNA of VRN2 was introduced into a middle vector pBPF？ 7.The fragment with P35S promoter and nos were digested by PstI,which were connected to pBI121 vector,giving plant espression vector containing VRN2 gene.