摘要
目的 为了通过细胞融合研制肺癌疫苗,需要建立有适合细胞融合的肺癌抗原细胞。方法 用8氮鸟嘌呤(8azaguanine,8 A G)从小鼠肺癌细胞 L A795 诱导抗8 A G 细胞株 A L A9702 ,并鉴定其生物学特性及成瘤性。结果 A L A9702 细胞在1.31×10- 4m ol/ L 8 A G 培养液中生长了1 年,在 H A T培养液中不能形成集落,并保持了来源细胞 L A795 的生长特性、染色体数目、成瘤性及肺转移性。 结论 A L A9702细胞保持了来源细胞的生物学性质及成瘤性质,是一株稳定的抗8 A G的小鼠肺癌细胞株,将作为适合细胞融合的抗原细胞,为研制肺癌疫苗,建立对肺癌有效的特异性免疫治疗奠定良好的基础。
Objective To preparing lung cancer vaccine by cell fusion technique, the lung cancer antigenic cell suitable cell fusion will have to be established. Methods An anti 8 azaguanine cell line ALA 9702 was induced by 8 azaguanine (8 AG) from mouse lung cancer cell line, LA 795 . The biological characteristics and carcinogenicity of the ALA 9702 cells have been appraised both in vivo and in vitro way. Results The ALA 9702 cells had been cultured over one year in medium containing 1 31×10 -4 mol/L 8 AG, and maintained their growing characteristics, chromosome number, carcinogenicity and metastasis of the original cells, LA 795 cells. Conclusion ALA 9702 cell maintained their biological characteristic and carcinogenicity of the original cells, LA 795 cells. It is a stable anti 8 AG mouse lung cancer cell line, and provides antigenic cell suitable for cell fusion to study lung cancer vaccine, which is the foundation for establishment of effective specific lung cancer vaccine therapy.
出处
《中国胸心血管外科临床杂志》
CAS
1999年第3期140-142,共3页
Chinese Journal of Clinical Thoracic and Cardiovascular Surgery
基金
国家自然科学基金
卫生部基金