复方地黄对老年性痴呆大鼠的行为学及脑内SOD、MAO的活性影响的实验研究

Study on Dihuang Compound on Alzheimer′s behavior in rats and brain SOD,MAO activity in rats

目的:研究应用复方地黄对阿尔茨海默病(Alzheimer′s Disease,AD)模型大鼠行为学的改善作用并探讨其与脑内SOD、MAO的关系。方法:在腹腔注射D-半乳糖造成大鼠衰老的基础上,海马注射喹啉酸(QA)造成拟老年性痴呆学习记忆损害模型,复方地黄治疗,观察Morris水迷宫检测大鼠学习记忆水平的行为学改变,生化检测脑内SOD、MAO的活性变化。结果:复方地黄可明显缩短AD大鼠的Morris水迷宫潜伏期、增加平台象限路程比和跨平台次数,降低脑内MAO水平,提高脑内SOD活性。结论:复方地黄对AD模型大鼠受损的神经行为学有明显的改善且与脑内SOD、MAO活性水平有关。

Objective： To explore the ethological improving effect of Dihuang Compound in treating model rats of Alzheimer＇s Disease （AD） and discuss the relation with SOD and MAO in rat brain. Methods： Injecting D-galactose in rats＇ abdominal cavity to doat them, then injecting quinolinie acid （QA） in rats＇ hippocampus to make the mode of senile dementia learning and memory impairment, given aqueous solution of Dihuang Compound by garage to observe the learning and memory ethological improving in Morris water maze and check the change of activity SOD and MAO in brain by bio- chemical test. Results： Dihuang compound can obviously reduce the latency of AD rats in Morris water maze, increase the platform quadrant journey ratio and the times of crossing platform, decrease the level of MAO and increase the activity of SOD in brain. Conclusion： Dihuang Compound can ameliorate the behaviour deficits of AD ratsmodels and related with the level od activity of SOD and MAO in brain.