摘要
从多穗柯中提取分离得到三叶苷,以抗糖尿病药物阿卡波糖为阳性对照,检测多穗柯三叶苷对小肠α-葡萄糖苷酶和胰脏α-淀粉酶活性的抑制作用和对α-葡萄糖苷酶活性抑制类型,并以芦丁为阳性对照检测其清除DPPH自由基能力。结果显示:多穗柯三叶苷对α-葡萄糖苷酶有明显抑制作用,为非竞争性抑制,抑制效果与阿卡波糖无明显差别,对α-淀粉酶活性的抑制率低于阿卡波糖;清除DPPH自由基能力强于芦丁。表明多穗柯三叶苷是一种有应用潜力的抗糖尿病活性物质。
The inhibitory effects of trilobatin separated from Lithocarpus polystachyus Rehd on the activities of α-glucosidase and α-amylase were determined and compared with those of the anti-diabetic drug acarbose as a positive control and the type of the enzymatic inhibition on α-amylase was investigated.Also,rutin was used as a positive control to measure the ability of the trilobatin from Lithocarpus polystachyus Rehd to scavenge DPPH free radicals.The results showed that α-glucosidase activity was inhibited remarkably by the trilobatin,and the inhibition was noncompetitive and did not exhibit a significant difference when compared to acarbose.Its inhibition rate against α-amylase,however,was lower than that of acarbose despite of having stronger DPPH free radical scavenging effect than rutin.From these experimental results,a conclusion could be drawn that the trilobatin from Lithocarpus polystachyus Rehd is a potential anti-diabetic compound.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2011年第5期32-35,共4页
Food Science
基金
广东省自然科学基金项目(8152800001000023)
