摘要
对五味子乙素进行清除自由基及体外抑菌实验。结果表明:五味子乙素对自由基的清除效果明显,其中对羟自由基清除作用大于相同质量浓度的VC,其半数清除质量浓度为0.2mg/mL;对超氧阴离子自由基清除作用小于VC,其半数清除质量浓度为0.24mg/mL。五味子乙素提取液对金黄色葡萄球菌、白色念珠菌、沙门氏菌、大肠杆菌、枯草芽孢杆菌均有一定的抑制作用,其中对白色念珠菌和金黄色葡萄球菌的抑制作用最强,最低抑菌浓度为0.25mg/mL,对根霉、黑曲霉、南洋酵母无明显抑菌活性。
Schisandrin B obtained from the fruits of Schisandra chinensis by supercritical carbon dioxide extraction and subsequent preparative HPLC separation was tested for its free radical-scavenging and antimicrobial activities in vitro.The results showed that Schisandra chinensis-derived schisandrin B had higher hydroxyl radical scavenging effect(IC50=0.2 mg/mL) but lower superoxide anion radical scavenging effect(IC50=0.24 mg/mL) when compared with vitamin C at the same concentrations.The compound could inhibit Staphylococcus aureus,Candida albicans,Salmonella,E.coli and Bacillus subtilis,especially against Candida albicans and Staphylococcus aureus with a minimum inhibitory concentration(MIC) of 0.25 mg/mL.However,no obvious inhibitory effect on Rhizopus,Aspergillus niger or Nanyang yeast was observed.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2011年第5期79-82,共4页
Food Science
基金
"十一五"国家科技支撑计划项目(2006BAI09B04)
沈阳农业大学青年教师基金项目(2010)
关键词
五味子
乙素
清除自由基
抑菌
Schisandra chinensis(Turcz.) Baill
schisandrin B
free radical-scavenging activity
antimicrobial activity