山核桃加工、贮藏前后总多酚含量及其抗氧化活性的变化

Changes in Total Polyphenols Content and Antioxidant Activity of Raw, Processed and Preserved Carya cathayensis Sarg Kernel

采用Folin-Ciocalteu法测定未加工、加工和贮藏的山核桃仁中总多酚的含量,同时研究山核桃仁总多酚的总抗氧化能力、还原力和对羟自由基的清除率。结果表明:未加工、加工和贮藏的山核桃仁提取液中总多酚质量浓度分别为1065.4、319.78mg/L和204.4mg/L。以2140mg/L的VE作为标准对照,总抗氧化能力和还原力:未加工山核桃>VE>加工山核桃>贮藏山核桃;羟自由基清除率:VE>未加工山核桃>加工山核桃>贮藏山核桃。总抗氧化能力、还原力和羟自由基的清除率与总多酚含量密切相关,总多酚是山核桃仁中重要的抗氧化物质。

The contents of total polyphenols total antioxidant activity,reducing power and hydroxyl radical scavenging capacity of 70% methanol extracts from raw,processed and preserved Carya cathayensis Sarg kernel were determined by Folin-Ciocalteu colorimetry and their total antioxidant activity,reducing power and hydroxyl radical scavenging capacity were measured.Total polyphenols contents were 1065.4,319.78 mg/L and 204.4 mg/L in the extracts from raw,processed and preserved Carya cathayensis Sarg kernel,respectively.The total antioxidant activity and reducing power of the three extracts as evaluated with 2140 mg/L vitamin E as a reference standard decreased sequentially in the following order： raw Carya cathayensis Sarg kernel vitamin E 〉processed Carya cathayensis Sarg kernel〉 preserved Carya cathayensis Sarg kernel.In the case of hydroxyl radical scavenging rate,the order was vitamin E 〉raw Carya cathayensis Sarg kernel 〉processed Carya cathayensis Sarg kernel 〉preserved Carya cathayensis Sarg kernel.Furthermore,total antioxidant activity,reducing power and hydroxyl radical scavenging rate had close correlation with the content of total polyphenols.Therefore,polyphenols are the important antioxidant components in Carya cathayensis Sarg kernel.