桑黄菌丝体多糖的分离纯化及理化性质分析

Isolation, Purification and Physico-chemical Characteristics Analysis of Mycelial Polysaccharides from Phellinus baumii

研究桑黄粗多糖的分离和纯化工艺,并对多糖组分进行理化性质分析。结果表明:经DEAE-52纤维素离子交换层析和Sephadex G-100凝胶过滤层析得到两个组分P-47000和P-8700;经高效液相色谱分析证明,两组分均为纯品,且不含蛋白质、核酸,为非淀粉类多糖,分子质量分别为4.74×104D和8.71×103D,均由鼠李糖、阿拉伯糖、木糖、葡萄糖、半乳糖组成,P-47000中各单糖物质的量比为3.47:1.99:1:63.27:13.44,P-8700中各单糖物质的量比为10.46:1:1.03:182.75:30.94。

The isolation,purification and physi-cochemical characterization of mycelial polysaccharides from Phellinus baumii were studied.Two polysaccharide fractions were separated from crude deproteinized polysaccharides from Phellinus baumii mycelia by DEAE-52 cellulose column chromatography,followed by purification on Sephadex G-100 gel filtration column.The two fractions were determined by HPLC to contain neither protein nor nuclear acid and to be pure non-starch polysaccharides,their molecular weights were 4.74×104 D and 8.71×103 D,respectively,and they were both composed of rhamnose,arabinose,xylose,galactose and glucose in a molar ratio of 3.47：1.99：1：63.27：13.44 and 10.46：1：1.03：182.75：30.94,respectively.