中国金顶侧耳菌株遗传多态性的ISSR分析

Genetic Polymorphism Analysis of Pleurotus cituinopileatus by ISSR

金顶侧耳是重要的食药用真菌。本实验收集全国各地18种金顶侧耳菌株,通过ISSR分子标记技术分析金顶侧耳菌株种内遗传多态性,11种引物扩增出102个条带,其中78个条带具有多态性,多态率为76.4%。结果表明,金顶侧耳菌株内具有丰富的遗传多样性,遗传相似系数在0.50～0.96。采用平均分类法UPGMA分析表明,在遗传相似系数为0.62时,18种菌株分为两大类。当遗传相似系数增大到0.75时,它们分为7类。16号菌株具有独立的遗传体系。

Pleurotus cituinopileatus is an important kind of edible medicinal fungi.Totally 18 kinds of Pleurotus cituinopileatus strains were collected from some markets in China.Inter-simple sequence repeat（ISSR） method was used to analyze genetic polymorphism of Pleurotus cituinopileatus strains.A total of 102 DNA fragments were amplified by using eleven ISSR primers,and 78 of these fragments were polymorphic with polymorphism rate of 76.4%.Meanwhile,high-level genetic polymorphism was observed in Pleurotus cituinopileatus and the genetic similarity coefficients（GSC） were between 0.50 and 0.96.These 18 strains were divided into 2 groups at GSC of 0.62 and 7 groups at GSC of 0.75 according to the cluster analysis of UPGMA method.Strain 16 had an independent genetic system.These results will provide a scientific fundament to accelerate genetic breeding and identification of cultivated strains of Pleurotus cituinopileatus.