部分稀土元素对灵芝多糖和三萜类物质液体发酵的影响

Effects of Some Rare Earth Elements on Liquid Fermentation of Ganoderma lucidum to Produce Polysaccharides and Triterpenoids

研究5种稀土元素(镧(La)、铈(Ce)、钕(Nd)、镨(Pr)、铒(Er))对灵芝液体发酵生产灵芝多糖产量和灵芝三萜类物质的影响。结果表明:不同的稀土元素在适宜浓度时对灵芝多糖和灵芝三萜的产量均具有一定程度的促进作用。其中稀土元素镨对灵芝胞内多糖、胞外多糖和胞内三萜的生产促进作用最明显,分别在浓度为0.01、0.001mmol/L和0.1mmol/L时,使三者产量分别达到最高的772.56、452.76mg/L和398.49mg/L,分别是对照的4.67、3.05和1.91倍;而对于胞外三萜,0.001mmol/L的镧诱导效果最好,其产量达到610.08mg/L,是对照的2.03倍。可见适宜的稀土元素及浓度可显著提高灵芝多糖和灵芝三萜的生产。

In this study,the effects of some rare earth elements such as La3＋,Ce3＋,Nd3＋,Pr3＋ and Er3＋ on the production of polysaccharides and triterpenoids by liquid fermentation of Ganoderma lucidum were explored.The results indicated that obvious stimulation effects of these rare earth elements on the production of polysaccharides and tritepenoids were observed.Moreover,Pr3＋ at concentrations of 0.01,0.001 mmol/L and 0.1 mmol/L revealed the most obvious improvement on the production of intracellular polysaccharides（IPS）,extracellular polysaccharides（EPS） and intracellular triterpenoids（IGT） with yields of 772.56,452.76 mg/L and 398.49 mg/L,respectively.Compared with the control group,three components were increased by 4.67,3.05 folds,and 1.91 folds due to Pr3＋ treatment.However,La3＋ at a concentration of 0.001 mmol/L showed a remarkable enhancing effect on EPS and the yield of EPS was up to 610.08 mg/L with a 2.03-fold enhancement.Therefore,the rare earth elements at appropriate concentrations can obviously increase the production of polysaccharides and triterpenoids in Ganoderma lucidum during liquid fermentation.