琐琐葡萄总黄酮在体外对乙型肝炎病毒的影响

Inhibitory Effects of Total Flavones from Vitis vinifer L. on Hepatitis B Virus in vitro

目的:考察琐琐葡萄总黄酮体外抗乙型肝炎病毒(HBV)的效果。方法:用HBV DNA转染人肝癌细胞所得的HepG 2.2.15细胞株为模型,ELISA法测定用药后8d细胞培养上清液中HBsAg和HBeAg含量,计算琐琐葡萄总黄酮对HBsAg和HBeAg分泌的抑制率,荧光定量PCR法测定HBV DNA的变化,MTT比色法检测细胞毒性。结果:琐琐葡萄总黄酮在12.5～100μg/mL范围内,均能不同程度地减少细胞培养上清液中HBsAg和HBeAg的含量,且细胞毒性小,半数中毒剂量(TC50)为284.91μg/mL,对HBsAg半数有效剂量(IC50)为327.56μg/mL、对HBeAg为215.34μg/mL,对HBsAg治疗指数(TI)为0.87、对HBeAg治疗指数为1.32;总黄酮质量浓度100μg/mL时对HBVDNA的抑制率为47.63%。结论:琐琐葡萄总黄酮具有一定的体外抗HBV作用。

Objective： To investigate the inhibitory effects of total flavones from Vitis vinifer L.on hepatitis B virus（HBV） replication and their toxicity in cultured human hepatocellular liver carcinoma cell line HepG 2.2.15 transfected with HBV DNA.Methods： After 8 days of treatment with total flavones from Vitis vinifer L.,the contents of HBsAg and HBeAg in the cell culture supernatant of HepG 2.2.15 were measured by ELISA to calculate the inhibitory rates against the secretion of HBsAg and HBeAg,the change in HBV DNA was monitored by real-time PCR,and cytotoxicity test was performed using MTT assay.Results： Addition of total flavones from Vitis vinifer L.in a dosage range of 12.5 to 100μg/mL could reduce the contents of HBsAg and HBeAg in the cell culture supernatant of HepG 2.2.15 to different extents.Little cytotoxic effect was observed with a median toxic concentration（TC50） of 284.91 μg/mL.The median effective doses（IC50） on HBsAg and HBeAg were 327.56 μg/mL and 215.34 μg/mL,and the therapeutic index for HBsAg and HbeAg were 0.87 and 1.32,respectively.Total flavones from Vitis vinifer L.at 100 μg/mL could inhibit HBV DNA by 47.63%.Conclusion： Total flavones from Vitis vinifer L.have inhibitory effects on HBV in vitro.