超高效液相色谱法测定侧柏炭中5个黄酮类成分

Determination of five flavonoids in Cacumen Platycladi Carbonisatum by UPLC

目的建立同时测定侧柏炭中杨梅素、槲皮苷、槲皮素、山柰酚和穗花杉双黄酮5种黄酮类化合物的超高效液相色谱(UPLC)分析方法。方法采用Waters Acquity UPLC系统,Waters BEH C18柱(50 mm×2.1 mm,1.7 mm);流动相为0.05%甲酸水溶液-甲醇,梯度洗脱;体积流量0.2 mL/min;柱温30℃;进样量2μL;检测波长360 nm。结果杨梅素、槲皮苷、槲皮素、山柰酚和穗花杉双黄酮在线性范围内均具有良好的线性关系(r≥0.999 5);平均回收率在96.85%～99.01%,RSD≤4.00%。结论 UPLC分离效果及重复性好,且快速、简便,可作为侧柏炭的质量控制方法。

Objective To establish a UPLC method for simultaneous determination of five flavonoids（myricetin,quercitrin,quercetin,kaempferol,and amentoflavone） in Cacumen Platycladi Carbonisatum.Methods The analysis was performed on a Waters Acquity UPLC system with a Waters BEH C18 column（50 mm×2.1 mm,1.7 mm）.The five flavonoids were separated with gradient mobile phase consisting of 0.05% aqueous formic acid and methanol.The temperature of column was 30 ℃,and the injection volume was 2 μL/min,detection wavelength was 360 nm.Results The five flavonoids including myricetin,quercitrin,quercetin,kaempferol,and amentoflavone had good linearity（r≥0.999 5） within the linear ranges.The average recovery rate was 96.85% — 99.01% with RSD≤4.00%.Conclusion The developed UPLC method is simple,sensitive and accurate and has the good repeatability in separation,which is available for the quality control of Cacumen Platycladi Carbonisatum.