胎牛血清对猪孤雌胚早期体外发育的影响

Effect of supplementation of FCS on the development of porcine parthenogenetic embryo in vitro

探讨了在猪孤雌胚体外发育的第3、4、5、6天分别添加胎牛血清(FCS)对孤雌胚发育能力的影响。结果,在体外培养的第5、6天添加10%FCS组得到的囊胚率和囊胚孵化率最高,第5天添加FCS组的囊胚细胞数最多。结果表明,FCS可以促进猪孤雌胚胎的早期体外发育。

The effect of supplementation of 10%fetal calf serum（FCS） on the development of pig parthenogenetic embryos in vitro were investigated.FCS was added into NCSU-23 on days 3,4,5 and 6 of in vitro culture of porcine parthenogenetic embryos.The result showed that the groups of supplementation of FCS on days 5 and 6 obtained the highest blastocyst rate and the highest hatching blastocyst rate,and the group of supplementation of FCS on day 5 gained the highest cell numbers of blastocysts.In conclusion,FCS can enhance the in vitro development of porcine parthenogenetic embryos.