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牛黄体中促黄体素受体剪接异构体的表达研究 被引量：1

Expression of luteinizing hormone receptor splice variants in bovine corpus luteum
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摘要 利用RT-PCR结合测序技术,对牛黄体中促黄体素受体(Luteinizing hormone receptor,LHR)在转录后通过选择性剪接而形成的剪接异构体进行鉴定,并比较不同剪接异构体编码蛋白质氨基酸序列的差异,利用RT-PCR对黄体中的LHR剪接异构体进行定量分析,为研究LHR剪接异构体在黄体中的功能奠定基础。结果表明,牛黄体中LHR通过选择性剪接产生了包括全长mRNA在内的6种剪接异构体,其中第11外显子5′端的266个碱基的缺失会使mRNA因发生移码突变而使翻译提前终止。实时定量PCR结果显示,发生外显子缺失的mRNA的表达量总量是全长mRNA表达量的1.94倍。 In this paper,RT-PCR and sequencing technique was employed to identify the splice variants of Luteinzing hormone receptor（LHR） in bovine corpus luteum.The difference of deduced protein sequence of the variants was compared.And the expression level of these splice variants was quantified by RT-PCR.The results indicated that six isoforms of LHR were identified,and variants lacking the first 266 of exon 11 generated a frame shift,and produced a premature codon,.The result of RT-PCR showed that total expression of B,C,D,E and F was 1.94 fold compared with A.
作者 熊秋宏 邓琼 于汪洋 高妍 马腾壑 姜昊 戴立胜 许艳丽 崔香顺 赵玉民 赵志辉 张嘉保
机构地区 吉林大学畜牧兽医学院 吉林大学实验动物中心 吉林省农业科学院
出处 《中国兽医学报》 CAS CSCD 北大核心 2011年第3期440-443,共4页 Chinese Journal of Veterinary Science
基金 国家科技支撑计划资助项目(2007BAD55B03) "863"计划资助项目(2008AA101010) 国家自然科学基金资助项目(30972100)
关键词 牛 黄体 LHR 剪接 RT-PCR bovine corpus luteum LHR splice RT-PCR
分类号 S823 [农业科学—畜牧学] Q78 [生物学—分子生物学]
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参考文献19

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二级参考文献2

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共引文献3

同被引文献11

  • 1Ascoli M, Fanelli F, Segaloff D L. The lutropin/cho- riogonadotropin receptor, a 2002 perspective[J]. En- doer Rev, 2002,23 : 141-174.
  • 2Hutchison J S,Nelson P B,Zeleznik A J. Effects of dif ferent gonadotropin pulse frequencies on corpus lute um function during the menstrual cycle of rhesus mon keys[J]. Endocrinology, 1986,119 : 1964-1971.
  • 3Duncan W C,McNeilly A S,Fraser H M,et al. Luteini- zing hormone receptor in the human corpus luteum: lack of down-regulation during maternal recognition of pregnancy[J]. Hum Reprod, 1996,11 : 2291-2297.
  • 4Duncan W C, Cowen G M, Illingworth P J. Steroido- genie enzyme expression in human corpora lutea in the presence and absence of exogenous human chorionic gonadotrophin (HCG) [J]. Mol Hum Reprod, 1999, 5 :291-298.
  • 5Gundermann T, Birnbaumer M, Birnbaumer L. Evi- dence for dual coupling of the murine luteinizing hor- mone receptor to adenylyldual coupling of the murine luteinizing hormone receptor to adenylyl cyclase and phosphoinositide breakdown and Ca^2+ mobilization[J]. J Biol Chem, 1992,267(7) :4479-4488.
  • 6Loosfelt H, Misrahi M, Atger M, et al. Cloning and se- quencing of porcine LH-hCG receptor cDNA: variantslacking transmembrane domain [J]. Science, 1989, 245:525-528.
  • 7Hipkin W R,Sanchez-Y J,Ascoli M. Agonis t- induced phos-phorylation of the luteinizing hormone/chorionic gonadotropin receptor expressed in a stably transfect- ed cell line [J]. Mol En-docrinol,1993, (7):823-832.
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  • 10Dodeigne C, Thunus L, Lejeune R. Chemiluminescence as a diagnostic tool[J]. Talanta, 2000, 51 (3) : 415- 439.

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中国兽医学报
2011年 第3期

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