桦褐孔菌多糖的抗肿瘤活性研究

Study on Anti-Tumor Activities of Polysaccharides from Inonotus obliquus

研究桦褐孔菌多糖对肿瘤细胞增殖的抑制和对荷瘤裸鼠的体内抑瘤作用。采用MTT法检测桦褐孔菌多糖对Jurkat和Daudi细胞增殖的影响,建立Jurkat荷瘤裸鼠模型,研究给药后对荷瘤裸鼠的抗肿瘤作用。表明桦褐孔菌多糖在体外具有直接杀死Jurkat和Daudi肿瘤细胞的作用,最高抑制率分别为62.29%和66.42%,具有良好的剂量-效应关系,同时具有显著的体内抗肿瘤活性,大小剂量对Jurkat荷瘤的抑制率分别为43.52%和57.48%,并且能显著提高裸鼠的脾脏指数,表明桦褐孔菌多糖具有显著的抗肿瘤活性和增强免疫功能。

The research aimed to study the anti-tumor effect of polysaccharides from Inonotus obliquus （IOP）on tumor cell proliferation in vitro and on tumor-bearing nude mice in vivo. The effect of polysaccharides from Inonotus obliquus （IOP） on tumor Jurkat and Daudi cell proliferation was measured by MTT assay. The model of jurkat tumor-bearing nude was established. The anti-tumor effect of IOP on tumor-bearing nude mice in vivo was studied. The result showed that lOP could kill jurkat and daudi tumor cell directly in vitro. The highest inhibition rate was 62.29% and 66.42%, respectively, which showed an excellent dose-response relation. Similarly, IOP also shown the obvious anti-tumor activities in vivo. The inhibition rate at low and high dose were 43.52% and 57.48% ,respectively. IOP increased obviously the spleen index. It was concluded that lOP had obvious anti-tumor activities and improved immunological function.