镰刀霉菌(Fusarium sp)JN158紫色素的提取及理化性质

Study on Extraction and the Physicochemical Property of Purple Pigment from Fusarium sp.JN158

研究了由镰刀霉菌(Fusarium sp)JN158发酵所产紫色素的提取条件和理化性质,结果表明:该紫色素的提取溶剂为体积分数95%乙醇溶液;紫色素在酸性条件下对温度,光照,柠檬酸和苯甲酸钠有较好的稳定性;最大吸收波长为310 nm和510 nm;对H2O2和Na2SO3敏感;蔗糖与葡萄糖有增色效果;金属离子Zn2+、Na+、Ca2+对色素稳定性无影响,K+、Mg2+、Fe3+、Al3+、Cu2+对色素影响较大。

The optimum conditions of extracting purple pigment from the culture of Fusarium sp JN158 were studied, and then the physicochemical properties were analyzed. The results showed that the purple pigment could be extracted with 95 % ethanol（pH ： 1）. Under the acid condition, the pigment was stable to heat, light, citric acid, and sodium benzonate, the maximum absorption wavelengh were 310nm and 510nm, which affected by H2O2 and Na2 SO3, Sucrose and Glucose had the hyperchromic effect; The pigment was influenced by Fe^3＋ , Al^3＋ , K^＋ , Cu^2＋ , Mg^2 ＋ , but Mg^2＋ ,Zn^2 ＋ ,Na^＋ , Ca^2 ＋ could not badly affect it at all.