金枝柳组织培养快速繁殖技术

Tchnology of Tissue Culture and Rapid Propagation of Salix x aureo-pendula CL.

在MS培养基中分别加入不同质量浓度的6-苄基氨基腺嘌呤（6-BA）和吲哚丁酸（IBA）进行金枝柳组织培养试验,探讨快速繁殖技术.结果表明：随着培养基中激素含量的增高,形成愈伤组织的量也增多;在MS＋1.0 mg/L 6-BA＋0.1 mg/L IBA培养基中的不定芽增殖数量最多（18.8）,无根苗在1/2 MS＋IBA 1.0 mg/L培养基上生根率最高（96%）;金枝柳试管苗移栽到腐殖土中成活率最高（97%）,河沙中成活率次之（76%）,旱田土上成活率最低（58%）.

The technology of tissue culture and rapidly propagation of Salix x aureo-pendula CL.was studied in MS medium plus 6-benzyladenine（6-BA） and indolebtyric acid（IBA）.The results showed：The more hormone content in medium,the more callus will be.The number of adventitious buds in MS ＋ 1.0 mg/L 6-BA＋0.1 mg/L IBA was more than other mediums.The good medium to induce root was 1/2MS＋IBA1.0mg/L（96% rooted ratio）.There was higher proportion of viability planted test-tube seedlings in the humus soil.