毛细支气管炎患儿血清γ干扰素、白细胞介素-4水平变化及其临床意义

Changes of Interferon-γ and Interleukin-4 Levels in Serum of Children with Bronchiolitis and Their Clinical Significances

目的探讨毛细支气管炎患儿血清γ干扰素(IFN-γ)、IL-4水平变化及其与病情严重程度的相关性。方法 1.采用ELISA法检测54例毛细支气管炎患儿治疗前后和随访2个月时血清IFNγ-、IL-4水平;2.将54例毛细支气管炎患儿按呼吸困难评分平分为轻度组、中度组和重度组,比较3组患儿血清IFNγ-、IL-4水平变化。同时采用ELISA法检测20例健康儿童血清IFN-γ及IL-4水平做比较。结果 1.毛细支气管炎患儿治疗前血清IFN-γ水平显著低于健康对照组(P<0.01),治疗后其水平升高,显著高于治疗前(P<0.01),但仍低于健康对照组(P<0.01)。治疗前血清IL-4水平显著高于健康对照组(P<0.01),治疗后其水平下降,显著低于治疗前(P<0.01),但仍高于健康对照组(P<0.01)。随诊2个月后,IFNγ-、IL-4水平接近健康对照组。2.轻度组血清IFN-γ水平显著高于中度组,中度组显著高于重度组(Pa<0.01);中度组血清IL-4水平显著低于重度组(P<0.01)。结论毛细支气管炎患儿血清INF-γ水平降低,IL-4水平升高,FN-γ/IL-4水平失衡,并与病情严重程度有关。

Objective To explore the changes of interferon-γ（IFN-γ）,interleukin-4（IL-4） and their correlation with the severity of bronchiolitis in children.Methods 1.IFN-γ and IL-4 levels in serum were measured by enzyme-linked immunosorbent assay in 20 healthy children（healthy control group） and 54 children with bronchiolitis before treatment,after treatment and after 2 months of follow-up.2.Fifty-four cases of bronchiolitis based on dyspnea criteria were divided into mild group,moderate group and severe group,and the levels of IFN-γ,IL-4 in the 3 groups were compared.The levels of INF-γ and IL-4 in 20 healthy children were measured as controls.Results 1.The level of IFN-γ in children with bronchiolitis before treatment was significantly lower than that in healthy control group（P 0.01）.The level of IFN-γ in children with bronchiolitis in post-treatment group was significantly higher than that in pre-treatment group,but still significantly lower than that in healthy control group（P0.01）.The level of IL-4 in children with bronchiolitis in pre-treatment group was significantly higher than that in healthy control group（P0.01）.The level of IL-4 in children with bronchiolitis in post-treatment group was significantly lower than that in pre-treatment group,but still significantly higher than that in healthy control group（P0.01）.The levels of IFN-γ and IL-4 after 2 months of follow-up were close to those in the healthy control group.2.The level of IFN-γ in mild group was significantly higher than that in moderate group,and in moderate group was significantly higher than that in severe group（Pa0.01）.The le-vel of IL-4 in moderate group was significantly lower than that in severe group（P0.01）.Conclusions Children with bronchiolitis showed decreased IFN-γ level and increased IL-4 level,so IFN-γ/IL-4 level was imbalanced,and which was correlated with the degree of severity of the illness.