黏液玫瑰单胞菌16S rRNA基因序列及耐药性分析

16S rRNA gene sequence of R. mucosa and its drug resistance

目的对常规方法不易鉴定的一株临床细菌进行16S rRNA基因序列分析,并探讨该细菌的耐药性。方法菌株分离培养后进行常规的鉴定及药物敏感性分析,同时选择细菌的16S rRNA基因为靶序列,进行PCR扩增并测序,测序结果与GenBank比对。结果 API 20NE板条(法国生物梅里埃公司)将该菌鉴定为嗜中温甲基杆菌;细菌的16S rRNA基因序列与黏液玫瑰单胞菌相似性为99.5%;细菌的药敏试验显示对三代头孢耐药性高。结论黏液玫瑰单胞菌引起的感染比较罕见,菌株对三代头孢的高耐药应引起临床重视;16S rRNA基因序列分析方法可以较好地鉴定常规方法难以鉴定的不典型及罕见菌株。

OBJECTIVE To identify a clinical atypical strains by 16S rRNA gene sequence analysis, and to explore its drug resistance. METHODS The strain was identified by routine methods and its antimicrobial susceptibility testing was carried out at the same time. The strain＇s 16S rRNA genes were chosen to PCR reaction, amplify, and sequencing. Nueleotide sequences were compared with GenBank data-base. RESULTS The API 20NE identified the organism as Methylohac-terium mesophilicum. The BLAST analysis indicated that the strain was R. mucosa and the similarity was 99.5 % the strain was high resistant to the third-generation cephalosporins. CONCLUSION Infections caused by R. mucosa are rare. More attention should be paid to its high resistance to the third-generation cephalosporins. The 16S rRNA gene sequences technology makes it Dossible to identify bacterial atypical strains.