不同贮藏条件对麻疯树种子生理生化和萌发的影响

Effect of Different Storage Condition on Seed Physiology and Chemistry Character and Germination of Jatropha curcas

采用室温、4、-5℃的贮藏温度和8.7%、4.7%的种子含水量设置6种组合,对麻疯树种子进行贮藏试验,分别于贮藏前、贮藏1个月和贮藏3个月测定不同贮藏条件下种子生理生化指标和萌发特性的变化。结果表明,随着贮藏时间的延长,麻疯树种子出现明显的劣变现象,一方面是贮藏物质的水解,表现为蛋白质含量降低、可溶性糖含量增加;另一方面是保护酶系统的破坏,呈现明显的膜脂过氧化,SOD活性降低,MDA含量和超氧阴离子自由基产生速率增加。最终结果是种子活力下降,发芽率降低。6种贮藏组合中,贮藏效果最好的是4号处理(温度4℃,含水量4.7%),其次是3号处理(温度4℃,含水量8.7%),最差的是1号处理(常温,含水量8.7%)。初步认为,采用4.7%的种子含水量和4℃贮藏条件贮藏麻疯树种子效果比较好。

The seed storage experiment of Jatropha curcas was carried out by designing six kinds of different combinations of temperature and seed moisture（temperature： room temperature,4℃,-5℃.seed moisture：8.7%,4.7%）.The physiology and chemistry indexes,germination rate of seed were tested before storage,storaged one month,storaged three months.The results indicated that the seed germination capacity decreased siginficantly with storage time extending.On the one hand,the storage substance of seed hydrolyzed,the protein content decreased and the soluble sugar content increased.On the other hand,the protection enzyme system of seed was destoryed with membrance lipid peroxidation,the SOD activity decreaseded,the MDA content and the generated rate of Superoxide anion free radical increased.Finally,the seed vigor and germination rate of seed declined.Among the six kinds of combinations,the best combination for seed longevity was No.4（temperature 4℃ and seed moisture 4.7%）,the second was No.3（temperature 4℃ and seed moisture 8.7%）,and the worst combination was No.1（the room temperature and seed moisture 8.7%）.The outcome of this study preliminarily indicated that the best storage condition of Jatropha curcas seed was seed moisture 4.7% and temperature 4℃.