穿孔素和颗粒酶在胃癌表达及临床意义研究

Expression of PFP and GrB in gastric cancer and their clinical significance

目的探讨胃癌病人穿孔素和颗粒酶的表达情况及其临床意义。方法泸州医学院附属医院普通外科2008-2009年采用免疫组织化学染色PV法,检测穿孔素(PFP)和颗粒酶(GrB)在胃切缘组织、癌旁组织、胃癌组织及相应转移淋巴组织中的表达情况,并结合临床、病理特征情况进行分析。结果 (1)GrB在切缘组织-癌旁组织-胃癌组织中阳性表达率的差异无统计学意义(P>0.05);(2)GrB(+)与GrB(-)胃癌组织所对应同一病人的胃切缘组织、癌旁组织GrB表达的差异有统计学意义(P<0.01);(3)以PFP细胞作为阳性对照,无论GrB表达与否,发现PFP蛋白在切缘组织、癌旁组织、胃癌组织胃上皮细胞的胞浆中均未见表达;(4)GrB在胃癌原发灶和淋巴结转移灶阳性表达的差异无统计学意义(P=0.130)。PFP在所有淋巴结转移癌细胞中为阴性表达;(5)GrB在胃癌组织的表达情况结合相应临床病理特征分析发现,GrB表达的原发肿瘤可能对肿瘤细胞的淋巴结转移起到抑制作用。结论 GrB(+)胃癌细胞与PFP非共表达,未能证实GrB(+)胃癌细胞具有淋巴样细胞分化表型。GrB可能参与胃癌发生的全过程,是胃癌形成的早期事件。

Objective To study express and clinical significance of PFP and GrB in patient with gastric cancer. Methods The expression of PFP and GrB in stomach incision tissue, peripheral tissue of gastric cancer, cancer tissue and corresponding tissues of lymphoid metastases was detected by immunohistoehemieal PV staining between 2008 and 2009 in the Department of General Surgery of Affiliated Hospital of Luzhou Medical College. The expression was analyzed combined with clinical and pathological features. Results （1）Comparing the expression of GrB （＋） in stomach incision tissue, peripheral tissue of gastric cancer and cancer tissue, there is no significant difference in the adjacent difference two groups （P〉 0.05）. （2）Comparing the expression of GrB in corresponding stomach incision tissue and peripheral tissue of gastric cancer for the the same patient with GrB （＋） and GrB （-） , there was statistically significant difference （P 〈0.01）. （3）Taking the PFP cells as positive control, it showed that the PFP protein showed no expression in the stomach incision tissue, peripheral tissue of gastric cancer and the cytoplasm of adjacent epithelial cells of gastric cancer tissues, whether the GrB （＋） expressed or not. （4）Comparing the expression of GrB in gastric cancer and lymph node metastasis, there is no significant difference （P=0.130）. The PFP expressed negative in all lymph node metastasis. （5）Analyzing the GrB expression in gastric cancer and the corresponding clinical and pathological features, it showed that the expression of GrB in primary tumor may play an inhibitory effect on lymph node metastasis. Conclusion Non-co-expression between GrB （＋） gastric cancer cells and PFP has not demonstrated that the GrB （＋） gastric cancer cells have lymphoid cells phenotype. GrB may be involved in the whole process of gastric cancer, which is an early event in the formation of gastric cancer.