新疆哈萨克族食管癌与HLA-G基因多态性

Relationship between HLA-G genetic polymorphism and susceptibility of esophageal carcinoma in Kazakh nationality in Xinjiang

目的研究免疫相关基因HLA-G多态性与新疆哈萨克族食管癌易感性的关系。方法采用病例-对照研究方法,调查新疆哈萨克族食管癌患者132例和非食管癌患者254例,应用聚合酶链反应（PCR）技术和聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术分别对HLA-G基因14bp缺失（rs16375）和HLA-G＊0105N（rs41557518）多态性进行检测,并比较2个位点不同基因型与食管癌发病风险的关系。结果 rs16375位点基因型为＋14 bp/＋14 bp、-14 bp/＋14 bp、-14 bp/-14 bp;病例组与对照组的基因型及等位基因频率分布差异有统计学意义（χ^2=7.54,P=0.02;χ^2=6.80,P=0.01）;携带-14bp/-14bp基因型的个体发生食管癌的危险性是＋14 bp/＋14 bp基因型的2.69倍（OR=2.69,95%CI=1.30-5.55）;rs41557518位点基因型为C/-、C/C、-/-;病例组与对照组的基因型及等位基因频率分布差异无统计学意义（χ^2=1.19,P=0.28;χ^2=1.13,P=0.29）;同时携带基因型-14bp/-14bp和C/C的个体发生食管癌的危险性是同时携带基因型＋14bp/＋14bp和C/C个体的2.82倍（OR=2.82,95%CI=1.32-6.04）。结论 HLA-G基因14bp缺失（rs16375）多态性与哈萨克族食管癌发生有关。

Objective To determine the association between human leukocyte antigen G（HLA-G）polymorphisms and the susceptibility of esophageal carcinoma in Kazakh nationality in Xinjiang.Methods A case-control study was conducted.The subjects included 132 Kazakh patients with esophageal carcinoma（EC） and 254 cancer-free controls.The polymorphisms of HLA-G gene 14bp deletion at rs16375 and 0105N at rs4155751 were genotyped by polymerase chain reaction（PCR） and polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP）,respectively.Results There were significant differences in the frequencies of rs16375 genotype and allele between cases and controls（χ2=7.54,P=0.02;χ2=6.80,P=0.01）.Compared with those who had ＋14bp/＋14bp genotype,the individuals with-14bp/-14bp genotype had a 2.69 fold（95%confidence interval：1.30-5.55） increased risk of developing EC.There was no statistical difference in the frequencies of rs41557518 genotype and allele between cases and controls（χ2=1.19,P=0.28;χ2=1.13,P=0.29）.Compared with those who had ＋14bp/＋14bp and C/C genotypes,the individuals with-14 bp/-14 bp and C/C genotypes had a 2.82 fold（95%CI：1.32-6.04） increased risk of developing EC.Conclusion HLA-G 14bp（rs16375） polymorphism is associated with the susceptibility of esophageal cancer.HLA-G＊0105N（rs41557518）polymorphism is not associated with the susceptibility of esophageal cancer in Kazakh nationality in Xinjiang.