大豆异黄酮对大鼠卵巢Bcl-2和Bax蛋白表达影响

Effect of soy isoflavone on levels of bcl-2 and bax in ovary of small female rat

目的通过动物实验研究,观察B-cell lymphoma 2(Bcl-2)蛋白和Bcl-2-associated X protein(Bax蛋白)在大鼠卵巢组织中的表达情况。方法选用2月龄Wistar雌性大鼠50只,按体重随机分成5组:空白对照组(基础饲料),大豆异黄酮低、中、高剂量组[含量分别为100、200、300 mg/(kg.bw)]、雌激素组(EC)(己烯雌酚0.5 mg/kg),实验周期为7周;采用酶联免疫吸附实验(ELISA)测定各组大鼠卵巢组织中Bcl-2蛋白和Bax蛋白水平。结果空白对照组、大豆异黄酮低、中、高剂量组、雌激素组Bcl-2蛋白浓度分别为(1.41±0.19)、(1.43±0.20)、(2.07±0.58)、(2.42±0.32)、(2.95±0.78)pg/mL,组间差异均无统计学意义;Bax蛋白浓度分别为(33.64±5.83)、(30.02±9.44)、(17.33±4.21)、(9.67±1.95)、(10.69±4.95)pg/mL,其中大豆异黄酮高剂量组与空白对照组比较差异有统计学意义(P<0.05)。结论大豆异黄酮可抑制大鼠Bax蛋白表达,对Bcl-2蛋白表达无影响。

Objective To study the effect of soy isoflavone on levels of B-cell lymphoma 2（Bcl-2）protein and Bcl-2 associated X protein（Bax） in small female rat′s ovary.Methods Fifty two-month-old female rats were randomly divided into five groups：control（baseline feed）,low dose（soy isoflavones of 100 mg/kg·bw）,moderate dose（soy isoflavones of 200 mg/kg·bw）,high dose（soy isoflavones of 300 mg/kg·bw）,and estrogen group（diethylstilbestrol of 0.5 mg/kg）.The treatment lasted for seven weeks.The levels of Bcl-2 and Bax were detected with ELISA.Results The gray values of Bcl-2 in control,low dose,moderate dose,high dose,and estrogen group were 1.41±0.19,1.43±0.20,2.07±0.58,2.42±0.32,and 2.95±0.78 without significant difference among the groups（P0.05 for all）and those of the Bax were 33.64±5.83,30.02±9.44,17.33±4.21,9.67±1.95,and 10.69±4.95,respectively.The soy isoflavone could remarkably reduce the activity of Bax in high dose group compared with that of the control group（P0.05）.Conclusion Isoflavone has aging-resistant effect on small female rat through antagonized pro-apoptotic bax protein and has no effect on Bcl-2.