酶法制备鮰鱼排水解蛋白的工艺优化

Study on optimization of preparing hydrolyzed animal protein from wastes (frames) of channel catfish,ictalurus punctatus

以斑点叉尾鮰鱼排为实验材料,在一定条件下,利用中性蛋白酶和风味蛋白酶进行复合酶解。实验分析了六个单因素(温度、初始pH值、料液比、加酶量、中性蛋白酶和风味蛋白酶的复合比、水解时间)对酶解效果的影响,然后采用Box-Behnken中心组合设计和响应面分析,确定了最佳的工艺条件为:温度50℃,初始pH值7.0,料液比1∶2’酶的复合比1∶1,酶解时间5h,加酶量2 000U/g原料。此时,鮰鱼排水解度(DH)可达28.87%。

The enzymatic hydrolysis of Channel catfish wastes（frames） with the complex of neutrase and flavourzyme was studied.The effects of several factors including hydrolysis time,solid-liquid ratio,quantity of enzyme,initial pH,hydrolysis temperature,the proportion of neutrase and flavourzyme were investigated.On the basis of single-factor experiments,the Box-Benhnken central composite design and response surface method were adopted to obtain the optimum conditions for enzymatic hydrolysis of Channel catfish wastes.The optimal enzymatic hydrolysis conditions were determined as：hydrolysis temperature 50℃,initial pH7.0,hydrolysis time 5.0 h,solid-liquid ratio 1：2,quantity of enzyme 2000 U/g and proportion of neutrase and flavourzyme 1：1.The degree of hydrolysis（DH） under optimum conditions could reach 28.87%.