复合不育剂EP-1对雄性长爪沙鼠的抗生育作用

Anti-fertility effect of EP-1 contraceptive compound on male Mongolia gerbils Meriones unguiculatus

在室内条件下采用食毒法研究了浓度为1/20 000的复合不育剂EP-1饵料对雄性长爪沙鼠性激素、生殖器官及繁殖行为的作用。结果显示:给药1周后,血清内促卵泡素(FSH)、促黄体素(LH)和雌二醇(E2)水平与对照组相比差异不显著,但睾酮(T)浓度显著下降;试鼠精囊腺重量显著降低;睾丸和附睾重量虽略有降低,但差异均不显著;给药组精子平均畸形率为(55.00±2.79)%,显著高于对照组的(32.00±2.36)%;给药组睾丸曲精小管平均异常率为(22.99±5.00)%,显著高于对照组的(4.21±1.18)%;试鼠配对后主要表现为相互嗅闻,给药组雄鼠主动嗅闻雌鼠频次显著低于对照组,打斗和攻击频次也降低;实验室条件下,40天内对照组繁殖率为55.6%,而给药组均未繁殖。说明EP-1对雄性长爪沙鼠具有明显的抗生育作用。

The effects of anti-fertility EP-1 at the concentration of 1/20000 on sex hormones, reproductive organs and reproductive behavior of male Mongolia gerbils were studied under the laboratory condition. One week treatment results had no significant differences in the concentrations of follicle-stimulating hormone （ FSH）, luteinizing hormone （ LH）, and exogenous estradiol-17-13 （E2） in serum by radioim- munoassay compared with the control. While the concentration of testosterone （T） was significantly lower than that of the control. The weight of seminal vesicle was obviously lower comparing with the control, hut no distinct differences had been found in the weight of testis and epididymis. The mean deformity rate of sperms was （55.00 ±2.79）%, which was two-fold higher than that of the control at （32.00± 2.36） %. The mean abnormal rate of the seminiferous tubules in the testis was （22.99 ± 5.00） % , which is higher than that of the control at （4.21 ± 1.18） %. The test mice in paired didn＇t mate but only smelled mutually, and the frequency of smelling was lower than that of the control, and the frequency of fighting and attacking also decreased. The reproductive rate of the control was 55.6% while the exper- imental group was zero.