重组DNA技术中可诱导的基因表达调控系统

Inducible gene expression and regulation systems in recombinant DNA technology

在重组DNA技术中,可诱导的基因表达调控系统可被用来调节目的基因的表达以达到基因功能研究、转基因动物研究、以及基因治疗研究等目的。该系统主要由诱导剂、可诱导的受体或转录因子、顺式作用元件以及载体系统四部分组成。本文以诱导剂为分类依据,叙述目前主要的6类可诱导的基因表达调控系统:类固醇激素受体诱导的基因表达调控系统、四环素诱导的基因表达调控系统、缺氧诱导的基因表达调控系统、高热诱导的基因表达调控系统、电离辐射诱导的基因表达调控系统和lac基因表达调控系统。

In recombinant DNA technology, inducible gene expression system can be used to regulate gene expression and achieve the purposes of research on gene function, transgenic animal, gene therapy, etc. These systems consists of four parts： inducer, inducible receptor or transcription factor, cis-acting element and the vector system. In this paper, we reviewed main 6 kinds of inducible gene expression systems, which are classified by inducers： steroid hormone receptor regulatory systems, tetracycline inducible gene expression system, hypoxia-induced gene expression system, heat-induced gene expression system, ionizing radiation-induced gene expression system and the lac gene expression system.