复方除痹汤治疗类风湿性关节炎的实验研究

The Empirical Study of FuFang ChuBi Decoction on Rheumatoid Arthritis

目的:探讨复方除痹汤对类风湿性关节炎的治疗作用及机制。方法:将76只SD大鼠随机分为6组,除正常组外其余各组大鼠均采用佐剂性关节炎大鼠模型,致炎30分钟前和致炎后8小时、22小时、32小时、46小时和56小时分别灌胃给药1次,正常组和模型组给予蒸馏水20 mL/kg(体质量,下同)灌胃,阳性组给予雷公藤多苷片40 g/kg灌胃,大、中、小剂量组分别给予复方除痹汤16 g/kg、8 g/kg和4 g/kg灌胃。停药至第17天观察继发性病变情况。第17天开始再继续灌胃给药,1次/d,剂量同上。每2天观察大鼠继发性病变情况并记录关节炎指数。致炎后第28天处死大鼠,取血并分离血清-20℃留存,进行血清TNF-αI、L-1β水平的检测。结果:与模型组相比,复方除痹汤大剂量组关节炎指数除了治疗第4天有差异外(P<0.05),其余时段均无差异(P>0.05);中剂量组除了治疗第6天无差异外,其余时段的关节炎指数都明显低于模型组(P<0.01);小剂量组在治疗过程中,各时段的关节炎指数均明显低于模型组(P<0.01);与模型组相比,阳性组和复方除痹汤大、中、小剂量组血清TNF-α及IL-1β水平均明显下降(P<0.01)。结论:复方除痹汤对佐剂性类风湿关节炎模型大鼠关节炎指数有一定的抑制作用;对类风湿关节炎模型大鼠异常升高的血清TNF-αI、L-1β水平具有显著的降低作用;复方除痹汤对类风湿性关节炎模型大鼠具有一定的治疗作用。

Objective：To explore the therapeutic effect and mechanism of FuFang ChuBi decoction on RA.Methods： 76 SD rats were randomly divided into six groups,besides the control group the left rats were adjuvant arthritis model.Rats were in 30 minutes before inflammatory and 8 hours,22 hours,32 hours,46 hours and 56 hours before and after inflammation respectively ig 1 time.The normal group and model group were given distilled water 20 mL/kg orally,the positive group were given Tripterygium glycosides tablet 40 mg/kg orally.And large,medium and low-dose group were given FuFangChuBi decoction 16 g/kg,8 g/kg and 4 g/kg.After 17 days,to observe the withdrawal of secondary lesions.In the 17 day oral administration to continue,1 time/d,dose as above.Every 2 days observed secondary lesions and recorded arthritis index.Inflamed rats were killed in the 28 days,and the blood was separated and retained in-20℃.To test the serum TNF-α,IL-1β levels.Results：Compared with model group,arthritis index of large dose of FuFan ChuBi decoction group had no difference except the 4th day after treatment（P0.05）;In addition to the 6th day after treatment, arthritis index of middle dose of FuFang ChuBi decoction was significantly lower than the model group（P0.01）;In the course of treatment,arthritis index of small dose of FuFang ChuBi decoction was significantly lower than model group（P0.01）.Compared with the model group,serum level of TNF-α of positive control group and large,middle and small dose of FuFang ChuBi decoction were significantly decreased（P0.01）;serum level of IL-1β of positive control group and large,middle and small dose of FuFang ChuBi decoction were significantly decreased（P0.01）.Conclusion：FuFang ChuBi decoction could inhibit the arthritis index of RA model rats;it could reduce serum levels of TNF-α,IL-1β of RA model rats.In a word,FuFang ChuBi decoction had a certain therapeutic effect on rheumatoid arthritis model rats.