金黄色葡萄球菌肠毒素A诱导的S180肉瘤细胞凋亡分析

Apoptotic Analysis of Sarcoma 180 Cells Induced by Staphylococcal Enterotoxin A

分析了金黄色葡萄球菌肠毒素A(SEA)诱导的S180肉瘤细胞凋亡。将S180肉瘤细胞接种于小鼠腋下,建立小鼠肿瘤模型,随机分为2组,即SEA实验组和生理盐水对照组,隔日进行腹腔注射,九天后解剖,将肿瘤组织做成石蜡切片。用末端转移酶介导的dUTP切口末端标记法(TUNEL)检测S180肉瘤细胞凋亡,并用免疫组化方法观察CD8+T细胞和颗粒酶B的分布,最后用ELISA法检测血清、脾脏中TNF-α的含量。SEA组的凋亡指数明显大于对照组,SEA组肿瘤组织周围的CD8+T细胞、颗粒酶B明显多于对照组,且血清、脾脏中TNF-α的含量也高于对照组。SEA诱导S180细胞凋亡与颗粒酶B和TNF-α的分泌有关。

To analyse the apoptosis of sarcoma 180 cells induced by Staphylococcal enterotoxin A（SEA）.A total of 40 mice were injected to the oxter by SEA to build up the tumor xenograft model,and then randomly divided into two groups.Physiologic saline（control） group and SEA（test） group were intraperitoneal injected every two days.Paraffin section was made after dissection nine days later.Apoptosis was detected by means of TdT-Mediated dUTP nick end labeling（TUNEL）.The distribution of CD8＋ T cells and granzyme B were analyzed immunohistochemically.In the mean time,TNF-α content was assayed by ELISA.There was an obvious difference of apoptotic index between SEA and control group.Both the CD8＋ T cells and granzyme B were found significantly more in SEA group than that in control group.Also TNF-α contents in serum and spleen were increased markedly.Apoptosis of sarcoma 180 cells induced by Staphylococcal enterotoxin A was related with granzyme B and TNF-α secretion.