摘要
为了制备赭曲霉毒素A多克隆抗体,采用戊二醛法合成赭曲霉毒素A-BSA人工抗原,通过紫外扫描法鉴定半抗原与载体蛋白偶联效果。合成的人工抗原用弗氏佐剂乳化后免疫Balb/c小白鼠,4次免疫后眼球下缘采血,分离血清。试验结果表明,免疫后产生了抗赭曲霉毒素A-BSA的特异性多克隆抗体,抗体效价为1:8100,赭曲霉毒素A的最低检出限为9 ng/mL。抗赭曲霉毒素A多克隆抗体的制备为开发赭曲霉毒素A的ELISA酶联免疫试剂盒奠定了基础。
To prepare polyclonal antibody against ochratoxin A,artificial antigen of ochratoxin A-BSA were coupled by glutaraldehyde method.The couple effection between semiantigen and carrier protein was identified through ultraviolet spectrum scanning method.The synthetical antigen ochratoxin A-BSA was mixed with the same amount adjuvant and then immunized mouse.After the fourth immunize,the mouse blood was collected from heart,and blood sera was separated.The experiment results showed that the specific polyclonal antibody against ochratoxin A was generated after immunity.The titer of anti-sera was 1：8100.The detection limit of ochratoxin A was 9 ng/mL.Preparation of the polyclonal antibody against ochratoxin A established the groundwork for further research of ochratoxin A immunoassay kit.
出处
《现代食品科技》
EI
CAS
2011年第3期324-327,336,共4页
Modern Food Science and Technology
基金
国家行业标准《食品中赭曲霉毒素的测定方法》(2009B164)
