3种不同中医证型艾滋病患者外周血CD_4^+CD_(25)^+调节性T细胞的表达

Expression of CD_4^+ CD_(25)^+ regulatory T cell in peripheral blood of HIV/AIDS patients with different TCM syndromes

目的:观察不同中医证型艾滋病患者CD4+CD2+5调节性T细胞(以下简称CD4+CD2+5 T细胞)的表达水平,探讨不同证型艾滋病免疫负调节能力。方法:根据艾滋病患者的症状、体征、舌象、脉象,按照中医辨证论治思路,分为肺肾不足、气虚血瘀、脾肾亏虚3种证型,设正常对照组30名,收集病例97例,观察不同证型艾滋病患者及正常对照组外周血CD4+CD2+5 T细胞的表达水平。结果:正常对照组、脾肾亏虚、肺肾不足、气虚血瘀各组患者CD4+CD2+5 T细胞的表达水平有统计学差异(P<0.001),脾肾亏虚型CD4+CD2+5 T细胞的表达水平最高,依次分别为肺肾不足型、气虚血瘀型。结论:不同证型艾滋病患者从病变初期的脾肾亏虚组,中期肺肾不足组,到疾病后期气虚血瘀组,CD4+CD2+5 T细胞的表达水平逐渐降低。

Objective： To observe the expression level of CD4＋ CD25＋ regulatory T cell in peripheral blood of HIV/AIDS patients with different TCM syndromes,and to probe the immune negative regulation ability of HIV/AIDS.Methods： Based on the symptoms,signs,tongue manifestations,pulse conditions of AIDS patients,according to the thought of TCM Treatment with syndrome differentiation,we divided the 97 HIV/AIDS patients into 3 different syndrome type,deficiency of pulmonary and renal syndrome,qi asthenia and blood stasis syndrome,and deficiency of spleen and renal syndrome,in order to observe the expression level of CD4＋ CD25＋ regulatory T cell in peripheral blood of HIV/AIDS patients with different TCM syndromes and 30cases of normal in control group.Results： The statistical significance of the expression level of CD4＋ CD25＋ regulatory T cell was observed among the 3 kinds of syndrome and control group（P0.001）.The deficiency of spleen and renal syndrome own the highest expression level of CD4＋ CD25＋ regulatory T cell,followed by deficiency of pulmonary and renal syndrome,and qi asthenia and blood stasis syndrome.Conclusion： The expression level of CD4＋ CD25＋ regulatory T cell of HIV/AIDS patients with different TCM syndromes has being reduced gradually from the deficiency of spleen and renal syndrome,to the deficiency of pulmonary and renal syndrome,and to the qi asthenia and blood stasis syndrome.