骨髓间充质干细胞复合聚乳酸-羟基乙酸材料治疗大鼠神经损伤的实验研究

Bone Marrow Mesenchymal Stem Cells Combined with Polylactic Glycolic Acid Scaffold for Rat Neural Injury

目的研究骨髓间充质干细胞（Bone Marrow Mesenchym＇,d Stem Cells，BMSCs）及其分化的神经样细胞分别与聚乳酸一羟基乙酸（polylactic glyeolie acid，PLGA）支架材料复合修复大鼠神经损伤的效果。方法将BMSCs复合PLGA培养，通过扫描电镜观察BMSCs在PLGA上的黏附、增殖并诱导分化成神经样细胞的情况；然后分别将单纯的PLGA、BMSCs—PLGA复合物以及神经样细胞一PLGA复合物分别移植入3组右侧坐骨神经损伤的SD大鼠模型中，术后观察三组动物60d。结果BMSCs及诱导分化生成的神经样细胞能在PLGA良好的黏附、增殖和生长；复合PLGA移植后能修复损伤的神经。结论BMSCs及神经样细胞复合PLGA移植可作为组织工程治疗脊髓损伤及糖尿病晚期神经源性膀胱的一种方法。

Objectives To study separately the effect of bone marrow mesenchymal stem cells （BMSCs） and differentiated neural -like cells combined with polylactic glycolic acid （PLGA） scaffolds, both composite which restore to SD rat nerve injury. Methods BMSCs were cultured by differential adherence, and composited PLGA, via scanning electron microscope to investigate the information of BMSCs＂ adhesion, proliferation and induction into neural -like cells in the scaffolds. Bold scaffold, BMSCs- PLGA complex, and neural -like cells- PLGA complex were separately transplanted into SD rat models, with the injury for right sciatic nerve, and obsvered SD rat models during 60 days after operation. Results BMSCs and neural - like cells can be exactly adhere to, proliferate and grow on the PLGA;BMSCs and neural -like ceils combined with PLGA, which can restore to nerve injury. Conclusions BMSCs and differentiated neural -like cells combined with polylactic glycolic acid （PLGA） scaffolds, which can be used as a method of tissue engineering, to treat ueurogenic bladder caused by spinal cord injury and advanced diabetes.