RT-PCR法特异性快速检测黄瓜绿斑驳花叶病毒

Rapid Detection of Cucumber green mottle mosaic virus by RT-PCR

根据黄瓜绿斑驳花叶病毒运动蛋白基因序列,设计并合成了特异性PCR检测引物,建立该病毒的快速检测方法。该方法可以从带毒叶片中扩增到约591 bp的片段,而TMV属其他9种病毒均无特异性扩增。本方法也适用于带毒种子的检测,具有准确、灵敏及时效性强等特点,为进出境植物检疫和农业安全生产提供可靠的技术支持。

In this study specific primers were designed based on the sequence of movement protein gene,a rapid and effective method for cucumber green mottle mosaic virus detection was established.The established detection system amplified band with expected size of 591 bp in infected leaves,but the other 9 viruses of Tobamovirus were not.The method,which can also detect CGMMV in seeds,is rapid,accurate and sensitive,providing a reliable technical support for entry-exit inspection and quarantine and safety of agricultural production.