青钱柳多糖降血脂作用的研究

Studies on Hypolipemic Effect of Cyclocarya paliurus(Batal) Iljinskaja Polysaccharide

通过体内和体外试验研究青钱柳多糖降血脂作用。胰脂肪酶活力测定法体外检测青钱柳多糖降血脂效果,高脂乳剂喂养小鼠2周造成高脂血症动物模型,尔后分组给药,多糖高剂量组(300 mg/kg)、中剂量组(200 mg/kg)、低剂量组(100 mg/kg)、辛伐他汀组(3 mg/kg)、辛伐他汀+多糖中剂量组(3 mg/kg+200 mg/kg)、模型组,持续喂养4周,每2周检测小鼠血清中胆固醇(TC)、甘油三脂(TG)、高密度脂蛋白(HDL-C)、低密度脂蛋白(LDL-C)的含量。结果表明,在体外试验中,青钱柳多糖对脂肪酶活性具有一定的抑制作用。动物试验表明青钱柳多糖能极显著地降低高脂血症动物的TC、LDL值(P<0.01);且在治疗高血脂时呈现一定的量效关系,以高剂量(300 mg/kg/d)最佳。降脂作用与辛伐他汀相当,且具有辅助辛伐他汀降脂的作用。

The hypolipemic effect of Cyclocarya paliurus（Batal） Iljinskaja polysaccharide was study by in vivo and in vtro experments.The hypolipemic effect of Cyclocarya paliurus（Batal） Iljinskaja polysaccharide was detected in vitro by measuring the activity of pancreatic lipase.A test model of mice was made according to lipid level of the animals.The animals were divided into 7 groups： the group with high dosages（H）（polysaccharide,300 mg/kg）,the group with medium dosages（M）（polysaccharide,200 mg/kg）,the group with low dosages（L）（polysaccharide,100 mg/kg）,the group of simvastatin treatment（ST）（3 mg/kg）,the group of simvastatin ＋ medium dosages treatment（ST＋M）（3 mg/kg＋ 200 mg/kg）,the model group with hyperlipidemia,and the normal group.All the mice were fed for 4 weeks.Then the influences of Cyclocarya paliurus（Batal） Iljinskaja polysaccharide on triglycerides（TG）,total cholesterol（TC）,low density lipoprotein cholesterol（LDL） and high density lipoprotein cholesterol（HDL）of serum in mice were determined every second week.Cyclocarya paliurus（Batal） Iljinskaja polysaccharide could inhibit the activity of pancreatic lipase in vitro.In the animal test,polysaccharide could remarkably reduce the level of serum TC and LDL（P〈0.01）,the effect of high dosages was the best,almost as effective as that of the group of sinvastatin treatment.All this suggests that Cyclocarya paliurus（Batal） Iljinskaja polysaccharide has good hypolipemic effect.