简易厌氧培养皿及厌氧罐检测厌氧菌的对比观察

COAGGREGATION AND COAGGREGATION INHIBITION BETWEEN FUSOBACTERIUM NUCLEATUM AND OTHER ORAL BACTERIA

在厌氧菌检验中，培养器材的选择是厌氧菌分离培养成败的关键之一。本室采用简易厌氧培养皿和厌氧罐两种不同器材进行对比试验，报告如下。１材料与方法１１器材简易厌氧培养皿为两个磨砂面边缘的玻璃平皿，高２ｃｍ，直径８５ｃｍ；厌氧罐选用高１７ｃｍ、直径１４ｃ...

The mode and ability of Fusobacterium nucleatum to coaggregate with other oral bacteria and their coaggregation inhibition are investigated in the present study. Method: the techniques of visual assay, spectrophotometric assay and transmission electron microscopy were applied to investigate this phenomenon. Results: It was proven that coaggregation occurred between specific partners. Fusobacterium. nucleatum could coaggregate with Porphyromonas gingivalis, Streptococcus. sanguis and Streptococcus mutans. Coaggregation bridge was also found when F. nucleatum, P.gingivalis and S. sanguis were mixed. The ability of F. nucleatum to coaggregate with both early and late colonizers suggested that it could serve as adherent bridge between these two groups of bacteria. When pretreated at 85℃ for 30 min, P.gingivalis, S.sanguis and S.mutans still showed nearly unchanged ability to coaggregate with unpretreated F. nucleatum, while F. nucleatum under the same pretreatment lost its ability to coaggregate with the rest of bactera which had not ben heated. Lactose and L-rhamnose could inhibit coaggregations above mentioned to a different degree. Conclusions: Coaggregation exits in the dental plaque ecosystem and coaggregation inhibition occurs under some pretreatment which offers theory of ecosystemetic prevention and therapy of periodontitis.